flow cytometry name Search Results


adenocarcinoma cell lines a549  (ATCC)
99
ATCC adenocarcinoma cell lines a549
Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) <t>A549,</t> (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.
Adenocarcinoma Cell Lines A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pax7 mouse monoclonal antibody igg2b  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank pax7 mouse monoclonal antibody igg2b
Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) <t>A549,</t> (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.
Pax7 Mouse Monoclonal Antibody Igg2b, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow particle image analyzer fpia-1000  (Sysmex Corporation)
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Sysmex Corporation flow particle image analyzer fpia-1000
Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) <t>A549,</t> (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.
Flow Particle Image Analyzer Fpia 1000, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crust flow® p2  (Air Liquide)
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Air Liquide crust flow® p2
Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) <t>A549,</t> (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.
Crust Flow® P2, supplied by Air Liquide, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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capillary flow pormeter model 1100 aex  (Porous Materials Inc)
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Porous Materials Inc capillary flow pormeter model 1100 aex
Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) <t>A549,</t> (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.
Capillary Flow Pormeter Model 1100 Aex, supplied by Porous Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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homopolypropylene h700  (Prime Polymer Co Ltd)
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Prime Polymer Co Ltd homopolypropylene h700
Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) <t>A549,</t> (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.
Homopolypropylene H700, supplied by Prime Polymer Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit antihuman mgrn1  (Proteintech)
93
Proteintech rabbit antihuman mgrn1
FIGURE 1 | The methylation level of <t>MGRN1</t> promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.
Rabbit Antihuman Mgrn1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry name  (Miltenyi Biotec)
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Miltenyi Biotec flow cytometry name
FIGURE 1 | The methylation level of <t>MGRN1</t> promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.
Flow Cytometry Name, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow tester cft-100  (Shimadzu Corporation)
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Shimadzu Corporation flow tester cft-100
FIGURE 1 | The methylation level of <t>MGRN1</t> promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.
Flow Tester Cft 100, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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np-kx-500 type mass capacity non-pulsation flow pump  (Nihon Seimitsu Co Ltd)
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Nihon Seimitsu Co Ltd np-kx-500 type mass capacity non-pulsation flow pump
FIGURE 1 | The methylation level of <t>MGRN1</t> promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.
Np Kx 500 Type Mass Capacity Non Pulsation Flow Pump, supplied by Nihon Seimitsu Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna lobind tubes eppendorf 86  (Eppendorf AG)
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Eppendorf AG dna lobind tubes eppendorf 86
FIGURE 1 | The methylation level of <t>MGRN1</t> promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.
Dna Lobind Tubes Eppendorf 86, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp ugt1a1 hs02511055 s1  (Thermo Fisher)
89
Thermo Fisher gene exp ugt1a1 hs02511055 s1
FIGURE 1 | The methylation level of <t>MGRN1</t> promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.
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Image Search Results


Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) A549, (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.

Journal: Frontiers in Immunology

Article Title: Single-cell mass cytometric analysis of peripheral immunity and multiplex plasma marker profiling of non-small cell lung cancer patients receiving PD-1 targeting immune checkpoint inhibitors in comparison with platinum-based chemotherapy

doi: 10.3389/fimmu.2023.1243233

Figure Lengend Snippet: Platinum-based chemotherapy and PD-1 blockade increased the production of tumor cell surface-specific IgG antibodies in well-responder NSCLC patients. The scatter plots demonstrate the effect of platinum-based chemotherapy (Chem., n=10) or second- line immunotherapy (ICI, n=10) to the level of IgG antibodies in the plasma of patients with reactivity of (A) A549, (B) H1975, or (C) H1650 NSCLC cell line cell surface epitopes. Age- and gender-matched healthy controls were recruited without known illness (Control, n=12). The experimental details can be found in the Materials and Methods. Briefly, flow cytometry was used to detect human NSCLC cell- line-specific cell surface binding IgG antibodies. *p < 0.05.

Article Snippet: Briefly, the human non-small cell lung cancer (NSCLC) cells, namely, adenocarcinoma cell lines A549, H1975, and H1650, were purchased from the American Type Culture Collection.

Techniques: Clinical Proteomics, Control, Flow Cytometry, Binding Assay

FIGURE 1 | The methylation level of MGRN1 promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.

Journal: Frontiers in oncology

Article Title: Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.

doi: 10.3389/fonc.2021.659254

Figure Lengend Snippet: FIGURE 1 | The methylation level of MGRN1 promoter was associated with platinum-resistant in HGSOC patients. (A) Schematic diagram of the abnormal methylation region (-1148 to -1064 upstream) in the promoter region of the MGRN1 gene. (B) The methylation of -1107 and -1097 CpG site were significantly hypermethylated in platinum-resistant HGSOC tissues compared with platinum-sensitive HGSOC tissues by MALDI-TOF Sequenom MassARRAY. (C) The mRNA expression of MGRN1 was lower in platinum-resistant HGSOC patients than platinum-sensitive HGSOC patients. (D) MGRN1 protein expression in HGSOC tissues. *P < 0.05, **P < 0.01.

Article Snippet: MGRN1 Immunohistochemical (IHC) Study of the Clinical Samples Of the 96 HGSOC samples, 52 paraffin-embedded HGSOC tissue samples collected in the pathology department of the Fourth Hospital of Hebei Medical University were used for immunohistochemical (IHC) staining ofMGRN1.MGRN1 immunostaining was performed using a primary antibody, namely rabbit antihuman MGRN1 (RNF156, 1:500 dilution; Proteintech, China).

Techniques: Methylation, Expressing

FIGURE 2 | The alteration of the sensitivity to cisplatin after a knockdown of MGRN1 expression in SKOV3 cells. (A) RT-qPCR assay showed the reduced expression of MGRN1 in shRNAa-MGRN1, shRNAb-MGRN1 and shRNAc-MGRN1 cells compared to shNC cells. (B) Western blot assay showed the reduced expression of MGRN1 in shRNAa-MGRN1 cells compared to shNC cells. (C) CCK-8 assays showed a significant increase in the proliferation rates in the shRNA- MGRN1 cells compared with the shNC cells after cisplatin treatment at several concentrations for 24h. (D) Flow cytometry showed that the apoptosis rate in the shRNA-MGRN1 cells was significantly lower than that in the shNC cells after exposure to cisplatin at the 10mM concentration for 24h. *P < 0.05, **P < 0.01. All the experiments were performed in triplicate.

Journal: Frontiers in oncology

Article Title: Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.

doi: 10.3389/fonc.2021.659254

Figure Lengend Snippet: FIGURE 2 | The alteration of the sensitivity to cisplatin after a knockdown of MGRN1 expression in SKOV3 cells. (A) RT-qPCR assay showed the reduced expression of MGRN1 in shRNAa-MGRN1, shRNAb-MGRN1 and shRNAc-MGRN1 cells compared to shNC cells. (B) Western blot assay showed the reduced expression of MGRN1 in shRNAa-MGRN1 cells compared to shNC cells. (C) CCK-8 assays showed a significant increase in the proliferation rates in the shRNA- MGRN1 cells compared with the shNC cells after cisplatin treatment at several concentrations for 24h. (D) Flow cytometry showed that the apoptosis rate in the shRNA-MGRN1 cells was significantly lower than that in the shNC cells after exposure to cisplatin at the 10mM concentration for 24h. *P < 0.05, **P < 0.01. All the experiments were performed in triplicate.

Article Snippet: MGRN1 Immunohistochemical (IHC) Study of the Clinical Samples Of the 96 HGSOC samples, 52 paraffin-embedded HGSOC tissue samples collected in the pathology department of the Fourth Hospital of Hebei Medical University were used for immunohistochemical (IHC) staining ofMGRN1.MGRN1 immunostaining was performed using a primary antibody, namely rabbit antihuman MGRN1 (RNF156, 1:500 dilution; Proteintech, China).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, shRNA, Flow Cytometry, Concentration Assay

FIGURE 4 | The high methylation of MGRN1 and its low mRNA expression are associated with poor survival in HGSOC patients. (A, B) Kaplan-Meier analysis of PFS and OS according to the MGRN1 methylation level in 26 HGSOC patients. (C, D) Kaplan-Meier analysis of PFS and OS according to the MGRN1 mRNA expression in 96 HGSOC patients.

Journal: Frontiers in oncology

Article Title: Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.

doi: 10.3389/fonc.2021.659254

Figure Lengend Snippet: FIGURE 4 | The high methylation of MGRN1 and its low mRNA expression are associated with poor survival in HGSOC patients. (A, B) Kaplan-Meier analysis of PFS and OS according to the MGRN1 methylation level in 26 HGSOC patients. (C, D) Kaplan-Meier analysis of PFS and OS according to the MGRN1 mRNA expression in 96 HGSOC patients.

Article Snippet: MGRN1 Immunohistochemical (IHC) Study of the Clinical Samples Of the 96 HGSOC samples, 52 paraffin-embedded HGSOC tissue samples collected in the pathology department of the Fourth Hospital of Hebei Medical University were used for immunohistochemical (IHC) staining ofMGRN1.MGRN1 immunostaining was performed using a primary antibody, namely rabbit antihuman MGRN1 (RNF156, 1:500 dilution; Proteintech, China).

Techniques: Methylation, Expressing

FIGURE 3 | The low mRNA expression of EGR1 was associated with platinum-resistant in HGSOC patients. (A) RT-qPCR showed the reduced expression of EGR1 in sh-MGRN1 cells compared to shNC cells. (B) The mRNA expression of EGR1 in platinum-resistant HGSOC patients and platinum-sensitive HGSOC patients. (C, D) Kaplan-Meier analysis of PFS and OS according to the EGR1 mRNA expression in HGSOC patients. *P < 0.05, **P < 0.01.

Journal: Frontiers in oncology

Article Title: Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.

doi: 10.3389/fonc.2021.659254

Figure Lengend Snippet: FIGURE 3 | The low mRNA expression of EGR1 was associated with platinum-resistant in HGSOC patients. (A) RT-qPCR showed the reduced expression of EGR1 in sh-MGRN1 cells compared to shNC cells. (B) The mRNA expression of EGR1 in platinum-resistant HGSOC patients and platinum-sensitive HGSOC patients. (C, D) Kaplan-Meier analysis of PFS and OS according to the EGR1 mRNA expression in HGSOC patients. *P < 0.05, **P < 0.01.

Article Snippet: MGRN1 Immunohistochemical (IHC) Study of the Clinical Samples Of the 96 HGSOC samples, 52 paraffin-embedded HGSOC tissue samples collected in the pathology department of the Fourth Hospital of Hebei Medical University were used for immunohistochemical (IHC) staining ofMGRN1.MGRN1 immunostaining was performed using a primary antibody, namely rabbit antihuman MGRN1 (RNF156, 1:500 dilution; Proteintech, China).

Techniques: Expressing, Quantitative RT-PCR